rabbit anti grp94  (Proteintech)

Journal: ACS Chemical Neuroscience

Article Title: Improving Proteostasis of Trafficking-Deficient GABA A Receptor Variants by Activating IRE1

doi: 10.1021/acschemneuro.5c00227

Figure Lengend Snippet: Mechanism of action of IRE1/XBP1s activators on enhancing GABA A R variant proteostasis. Certain GABA A R variants misfold in the ER and undergo excessive ER-associated degradation (ERAD), leading to their trafficking deficiency to the plasma membrane. IRE1/XBP1s activators enhance the ER folding capacity to promote the folding of a variant by increasing the interaction between the variant and BiP, a pro-folding chaperone, and further promote the assembly into pentameric receptors in the ER membrane. In addition, such IRE1/XBP1s activators inhibit the ERAD of the variant by decreasing the interaction between the variant and Grp94, Hrd1-Sel1L, and VCP, known ERAD factors for GABA A Rs. Consequently, IRE1/XBP1s activators enhance the anterograde trafficking of the variant to the plasma membrane by increasing the interaction between the variant and LMAN1. The receptor variant is also stabilized after reaching the plasma membrane.

Article Snippet: The rabbit polyclonal anti-Grp94 antibody (#14700-1-AP, 1:250) was obtained from Proteintech.

Techniques: Variant Assay, Clinical Proteomics, Membrane

Journal: bioRxiv

Article Title: Loss of SMARCB1 evokes targetable epigenetic vulnerabilities in Epithelioid Sarcoma

doi: 10.1101/2024.09.18.613695

Figure Lengend Snippet: (a) Western Blot of clonal EpS cell line re-expressing SMARCB1. (b) Western blot of cytosolic, nuclear and BRG1-coIP‘d cell lysates against BRG1, GRP94 (cytosolic), Lamin A/C (nuclear) and SMARCB1 in VA-ES-BJ. (c) GSEA-based network analysis of up-/down-regulated gene sets upon SMARCB1 re-expression from shared regulated genes in VA-ES-BJ and NEPS. (d) DOX dosage-dependent gradual loss of clonogenicity in VA-ES-BJ and NEPS: Exemplary wells and fitted clonogenicity response curves. (e) Delayed G1/S-phase cell cycle progression upon SMARCB1 re-expression in VA-ES-BJ and NEPS. (f) In vivo tumor growth curves of VA-ES-BJ s.c. xenografts with empty vehicle or clonal SMARCB1 re-expression. (g) Comparison of HE-stained patient and xenograft tumour tissue.

Article Snippet: Antibodies were used to detect SMARCB1 (Cell Signaling, #91735, 1:1,000) or GRP94 (Cell Signaling, #20292, 1:1,000) by reaction with a secondary HRP-conjugated, monoclonal murine, anti-rabbit antibody (sc-2357 1:2,000, Santa Cruz).

Techniques: Western Blot, Expressing, In Vivo, Comparison, Staining